منابع مشابه
Crystalline Ribonuclease
1. A crystalline enzyme capable of digesting yeast nucleic acid has been isolated from fresh beef pancreas. 2. The enzyme called "ribonuclease" is a soluble protein of albumin type. Its molecular weight is about 15,000. Its isoelectric point is in the region of pH 8.0. 3. Ribonuclease splits yeast nucleic acid into fragments small enough to diffuse readily through collodion or cellophane membra...
متن کاملCrystalline semisynthetic ribonuclease-S'.
Crystals of solid phase-derived semisynthetic ribonuclease-S' were prepared and compared with those for native ribonuclease-S' and -S. The semisynthetic species used was the noncovalent complex of synthetic fragment-(1-20), corresponding to residues 1 through 20 of bovine pancreatic ribonuclease-A (ribonucleate 3'-pyrimidino-oligonucleotidohydrolase, EC 3.1.4.22), and native ribonuclease-S-(21-...
متن کاملProteolytic Contaminants of Crystalline Ribonuclease
1. The ability of crystalline ribonuclease to hydrolyze proteins is due to impurities present in the preparations and not to an intrinsic property of the ribonuclease molecule. The amounts of these impurities vary enormously with different preparations. 2. The dangers inherent in the use of crystalline enzymes as specific tools, without assaying them for all possible interfering impurities, are...
متن کاملMolecular Weight and Electrophoresis of Crystalline Ribonuclease
Electrophoretic studies on purified crystalline ribonuclease showed the absence of any impurities differing in mobility from the bulk of material. The isoelectric point of ribonuclease was found by electrophoresis to be at about pH 7.8. Ultracentrifuge studies indicated fair homogeneity of ribonuclease in solution. Only one moving component has been observed. The molecular weight of ribonucleas...
متن کاملA Method for the Preparation of "protease-free" Crystalline Ribonuclease
A method is described for the preparation of crystalline ribonuclease free from all measurable traces of proteolytic enzymes.
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1950
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)56373-0